High-Throughput Detergent Screening for Membrane Protein solubilization using nanoliters of sample

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Poster

High-Throughput Detergent Screening for Membrane Protein solubilization using nanoliters of sample

Authors

I Moraes¹; A Danson²; R Marabini³;
¹ National Physical Laboratory, UK; ² National Physical Laboratory (NPL), UK; ³ Fida Biosystems ApS, Denmark

Discussion

Authors

I Moraes¹; A Danson²; R Marabini³;
¹ National Physical Laboratory, UK; ² National Physical Laboratory (NPL), UK; ³ Fida Biosystems ApS, Denmark

Discussion

In this work, we demonstrate how Flow Induced Dispersion Analysis (FIDA) is employed for high throughput detergent screening to assess the degree of solubilization and polydispersity of a GFP-tagged membrane protein. The entire screen of 12 detergents takes less than 3 hours and is performed directly in unpurified cell lysates, using only 1,5 uL. The sample tray is kept at 5 ⁰C for the duration of all measurements. We compare the FIDA detergent screening with Fluorescence Size-Exclusion Chromatography (FSEC). The comparison reveals that FIDA decreases the screening time from days to hours and leaves most of the membrane protein available for other experiments.