Authors

SR Kerr¹; 
¹ Singleron Biotechnologies, Germany

Discussion

High-throughput screens are an essential component of drug discovery and development. RNA-seq can be used to generate comprehensive information on how drugs effect the transcriptome, which then can be used to determine both the efficacy and mechanism of action for a compound. However, RNA-seq library preparation is a costly and tedious procedure, making it difficult to scale up RNA-seq experiments in high-throughput drug screens.

To overcome these challenges we have developed AccuraCode^®, a high-throughput RNA-seq library construction method that drastically reduces time and costs compared to traditional RNA-seq. Cell cultures from 384-well plates are barcoded in-plate, followed by a one-step whole transcriptome amplification to yield the cDNA with unique sample barcodes. Since 384 samples can be processed simultaneously in 1 library preparation reaction, the hands-on time and expenses are reduced by more than 90%. Thus, AccuraCode^® provides a fast, cost-effective high throughput method which could greatly facilitate the drug discovery process.