Authors

A Azimi¹; Z Zhang¹; J Wang¹; G Pengfei¹; Q Zhang¹; X Zou¹; Q Gao¹; M Qiu¹; H Cui¹; P Jiang¹; W Ju¹; Y Ji¹; P Pan¹; F He¹; 
¹ Pharmaron, UK

Discussion

Introduction: Automated microscope based High Content Screening (HCS) or High Content Imaging (HCI) has enabled scientists across academia and industry to simultaneously analyze many features in complex biology systems. Pharmaron have multiple sites of Operetta® CLS™ and IncuCyte S3 Live cell system and experienced in different types of readout to support evaluate cell phenotype, biomarkers, and biological program. IncuCyte S3 Live cell system also enable the real time reading. Multiple methods can be applied to evaluate cell proliferation, not limited to confluence and spheroid size, all the objects could be counted using proper dye or detection reagents to draw the treatment impact on cell growth during short or long period. Evaluation of DNA content also provide alternative way to evaluate cell cycle. It avoids complicated operation and enable higher reading throughput. With new generation of detection reagent, different cell progress including apoptosis and cytotoxicity effect in kinetic mode. These readouts can able be properly combined to traditional parameters to strengthen high content screening capability. Immunofluorescence is utilized to evaluate biomarker expression or localization. Operetta® CLS™ system provide high resolution of imaging and sophisticated analysing software to facilitate the standardized protocol of image analysis. Summary: Pharmaron has established assays to evaluation protein expression or modification in different cellular fraction and substructure formation. High Content Screening is widely used in Pharmaron to support preclinical drug discovery pipeline and made significant milestones for sponsors.