Developing Stable cell lines with Combinatorial Kv subtypes for Drug discovery.

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Poster

168

Developing Stable cell lines with Combinatorial Kv subtypes for Drug discovery.

Authors

K Osborn¹; JR Booth¹; M Barri²; DJ Field¹; R Murrel-Lagnado²; H Choudhury¹;
¹ Sussex Drug Discovery Centre, University of Sussex., UK; ² University of Sussex, UK

Discussion

Authors

K Osborn¹; JR Booth¹; M Barri²; DJ Field¹; R Murrel-Lagnado²; H Choudhury¹;
¹ Sussex Drug Discovery Centre, University of Sussex., UK; ² University of Sussex, UK

Discussion

There is remarkable diversity of Potassium channel (Kv) subtypes which is further increased by the ability of subunits to mix and match and form heterotetramers with different biophysical properties. We have established cell lines using an inducible bicistronic vector system in HEK293 cells that allow for the expression of two different Kv subunits. These cell lines behave in a manner predicted from known literature and would make a valuable tool both for exploring the biology of Kv channels and as a cell line for screening purposes.