Overview

Physiologically relevant in vitro assays that accurately mimic the tumour microenvironment are crucial for advancing immuno-oncology research. While 2D cell models are routinely used in research due to their simplicity and low cost, the lack of tumour architecture or extracellular matrix (ECM) components in these models limits their biological relevance, which impacts the translation of in vitro data through to the clinic. Current 3D cell models based on naturally derived matrices offer a higher

Introduction

Physiologically relevant in vitro assays that accurately mimic the tumour microenvironment are crucial for advancing immuno-oncology research. While 2D cell models are routinely used in research due to their simplicity and low cost, the lack of tumour architecture or extracellular matrix (ECM) components in these models limits their biological relevance, which impacts the translation of in vitro data through to the clinic. Current 3D cell models based on naturally derived matrices offer a higher

Methods

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Results

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Conclusion

Here, we describe two different 3D cell culture models to study immune cell association with tumour cells, immune cell-mediated anti-tumour effects, and immune cell infiltration into the matrix. The generation of these immuno-oncology models using the RASTRUM Platform enables: ● Compatibility with both tumour cell and immune cell culture to allow for the creation of co-cultures that recapitulate the tumour microenvironment ● Tunability of the matrix to modulate composition and stiffness to influe