Overview

RepliGut® Planar is a stem cell-derived platform that recreates the human intestinal epithelium and enables biologically relevant screening of compounds and disease modeling. The transwell format allows for easy apical and basolateral access for compound addition or supernatant analysis.

Introduction

Inherent variability in stem cell-based complex cultures interferes with the ability to build and validate robust assays that query drug responses and pharmacokinetics, especially in 3D organoids. RepliGut® Planar is an adult stem-cell derived intestinal model which, unlike organoids, is initiated by directly thawing cells into a transwell format and differentiating in situ. We aimed to identify and minimize sources of variability in the RepliGut® Planar platform.

Methods

Transverse colon epithelial cells from passages 2, 5, 10 and 15 were plated on transwell plates and TEER was measured every 24 hours in culture. RNA was isolated from cultures grown to day 3 in RepliGut® Maturation Media.

Results

Data shown on poster.

Conclusion

RepliGut® Models recreate critical features of the human intestinal epithelium and represent a physiologically relevant system to study inflammatory and toxicity responses in vitro across different human populations. By identifying and controlling sources of experimental variability, true donor difference in response to stimuli be detected.